Farnesylation antibody
WebAntibody Validation Research Associate II Cell Signaling Technology (CST) Apr 2011 - ... Protein farnesylation inhibitors cause donut-shaped cell nuclei attributable to a centrosome separation defect Web丁香通为您提供LMNALaminACA商品详情介绍：价格：￥4526，货号：LM14386，品牌：Leading Biology，产地：美国，详见丁香通LMNALaminACA商品详情页；
Farnesylation antibody
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WebFeb 15, 2014 · Employing a farnesylation-specific monoclonal antibody that we generated, we established by immunoprecipitation that the vast majority, if not all, of the endogenous LKB1 is prenylated. Levels of LKB1 localized at the membrane of the liver of LKB1C433S/C433S mice and their fibroblasts were reduced substantially compared with … WebJun 15, 1994 · A polyclonal antibody [anti-prelamin A antibody (alpha-PA)] has been obtained against the peptide LLGNSSPRTQSPQN which is proteolytically removed …
WebObjective: 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors (statins) can affect post-translational processes, thus being responsible for decreased farnesylation and geranylgeranylation of intracellular small G proteins such as Ras, Rho and Rac, essential for cell survival and proliferation. In this regard, recent in vitro and in vivo … Web(ab199481) Anti-Pan-Farnesylation antibody [EPR19409] Supplier Abcam. Host Rabbit Type Primary Clonality Recombinant (EPR19409) Recombinant Yes Conjugate Unconjugated Modification Unmodified View Product On Supplier's Website Request a Quote from Abcam. Add to Procurement List
WebJun 23, 2024 · Farnesylation of Rheb is essential for nucleotides loading and activation 38. ... Primary antibodies were diluted in 3% NGS-PBS and incubated overnight at 4 °C, followed by incubations with the ... WebJul 25, 2016 · Farnesylation was detected using polyclonal rabbit anti-Farnesyl antibodies (1:500 dilution; Affinity BioReagents). Detection was performed using ECL prime reagents (GE Healthcare Life Sciences ...
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WebApr 1, 2000 · Farnesylation of H-ras and K-ras-4B in vitro by purified human FT is inhibited by SCH66336 with IC 50 values of 1.9 and 5.2 n m,respectively. ... Generation and characterization of the monoclonal anti-PxF antibody will be described in greater detail elsewhere. 4 Affinity-purified peroxidase- and fluorochrome-coupled secondary … chuck reese obituaryWebJul 27, 2024 · The primary antibody 6E10 was used for assessing Aβ deposition, and IBA-1 antibody was used for assessing activated microglia. Detailed information on antibodies is available in Table 2 . Immunoreactivity of Aβ and IBA-1 in cortical and hippocampal areas was quantified using Image-Pro Plus (MediaCybernetics, Rockville, MD) and Fiji/Image J. chuck ramsey nflchuck stalls flood insuranceWebFeb 28, 2006 · As a control, the protein yield is tested by Western blotting with anti-GST- (or anti-HA-) antibodies on the same membrane that has been previously used for TLC … chuck d worthWebJul 26, 2010 · AnkB localizes to the LCV membrane through farnesylation of its CaaX motif. (A) The U937 cells and D. discoideum were infected and analyzed at 2 h by confocal microscopy using anti–L. pneumophila (Lpn) antibodies (green) and anti-AnkB antisera (red). Arrowheads indicate localization of AnkB with L. pneumophila. chuck snow photographyHuman RMS cell lines SMS-CTR and RD were obtained from American Type Culture Collection (ATCC). RH36 was provided by Dr. David Loeb (The Children’s Hospital at Montefiore, Bronx, New York), and CCA, RMS-YM, RH18, JR1 cell lines were provided by Dr. Marielle Yohe (National Institutes of Health, Rockville, … See more Immunoblotting was performed as described previously [68]. Cells were plated at 2 × 106cells per well in 10 cm plates and incubated … See more Cytosolic and membrane fractions were prepared per the manufacturer instructions (Thermo Scientific, #78840). Protein concentrations were determined using Pierce BCA Protein … See more RAF1-RBD immunoprecipitation was performed as previously described [69]. Cells were seeded in 10 cm dishes. The following day, the … See more RMS cell lines were plated at a density of 1 × 105 cells on glass coverslips in 6-well plates. Cells were incubated in 1000 nM tipifarnib for 24 h … See more chuck thibeaultWebFarnesylation is a post-translational modification . ... besides proteomic approaches, an anti-farnesyl antibody will certainly help you on in vitro and in vivo studies. You may also … chuck schumer relation to amy